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Price $276.00
Category NameCardiac Markers ELISA kits
MethodELISA: Enzyme Linked Immunosorbent Assay
PrinciplePeroxidase - Conjugated Competitive ELISA
Detection Range0-400ng/ml
Sample25 ul serum
Sensitivity0.5 ng/mL
Total Time~ 45 min
Shelf Life18 months from the manufacturing date

Item #:                    1005-15   Quantity:               



CK-MB ELISA Test kit

CK-MB ELISA Test kit Intended Use
CK-MB ELISA test kit is intended for the quantitative determination of Circulating Creatinine Kinase (CK-MB Isoform) concentrations in human serum.

CK-MB ELISA Kit Components:
• Microplate: 96 wells coated with streptavidin
• CK-MB Enzyme Reagent
• CK-MB Calibrators
• Wash Solution
• Substrate A
• Substrate B
• Stop Solution (1N HCL)

Method Principle
This Diagnostic Automation/ Cortez Diagnostics Inc. Human CK-MB ELISA test kit is based on the Enzyme Linked Immunosorbent Assay. In this ELISA, the immobilization takes place during the assay at the surface of a microplate wells through the interaction of streptavidin coated on the well and exogenously added biotinylated monoclonal anti-CK-MB antibody. For further details please refer to complete instructions manual supplied with the product.

Background Information
Creatinine kinase (CK) is an enzyme found primarily in muscle and brain tissue, which exists as three dimeric isoenzymes: CKMM (CK- 3), CK-MB (CK-2), and CK-BB (CK-1), built from subunits M and B. The CK-MB isoenzyme, accounts for 5 to 50% of total CK activity in myocardium. Serial measurements of biochemical markers is now accepted universally as an important determinant in ruling in or ruling out acute myocardial infarction. CK-MB is one of the most important myocardial markers with well-established roles in confirming acute myocardial infarction (AMI) and in monitoring reperfusion during thrombolytic therapy following AMI. In AMI, plasma CK-MB typically rises some 3 to 8 hours after the onset of chest pains, peaks within 9 to 30 hours and returns to baseline levels within 48 to 72 hours. The pattern of serial CK-MB measurement is more informative than a single determination. Accordingly it has been recommended that CK-MB be measured on admission to the emergency room, and at regulated intervals thereafter.

For more information about ELISA Kits, Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representative at 818-591-3030.