Description

Anti-Phospholipid Screen IgG/IgM ELISA Test kit





Anti-Phospholipid Screen IgG/IgM kit Intended Use
Human Anti-Phospholipid Screen IgG/IgM kit is a quantitative enzyme immunoassay (EIA) intended to screen for the presence of IgG and IgM class autoantibodies against Cardiolipin, Phosphatidyl Serine, Phosphatidyl Inositol, Phosphatidic acid and Beta2-Glycoprotein I in human serum or plasma as an aid in the diagnosis of thrombosis in patients with Systemic Lupus Erythematosis (SLE) or lupus-like disorders.





Kit Components:
• Microplate: 96 wells coated with a mixture of highly purified phospholipids
• Standards: 6 vials each containing Combined Anti-phospholipid Calibrators:
IgG: 0; 6.3; 12.5; 25; 50; 100 GPL U/ml
IgM: 0; 6.3; 12.5; 25; 50; 100 MPL U/ml
• Control: 2 vials, each containing Anti-phospholipid Controls
• Sample Buffer Concentrate (5x)
• Enzyme Conjugate IgG: containing polyclonal anti-human IgG-HRP
• Enzyme Conjugate IgM: containing polyclonal anti-human IgM-HRP
• TMB Substrate Solution
• Stop Solution (1 M hydrochloric acid)
• Wash Solution Concentrate (10X)





Method Principle
This Diagnostic Automation/ Cortez Diagnostics Inc. Human Anti-Phospholipid Screen IgG/IgM ELISA Test kit is based on the principle of ELISA- Indirect assay using a mixture of highly purified phospholipids coated microwells. Antibodies against these antigens, if present in diluted serum or plasma, bind to the respective antigens. For further details please refer to complete instructions manual supplied with the product.





Background Information
Screening for the presence of IgG and IgM class autoantibodies against Cardiolipin,Phosphatidyl Serine, Phosphatidyl Inositol, Phosphatidic acid, and Beta2- Glycoprotein I in human serum or plasma is an aid in the diagnosis of thrombosis in patients with Systemic Lupus Erythematosis (SLE) or lupus like disorders. SLE or lupus is a systemic autoimmune diseases that can affect any part of the body. The immune system attacks the cells and tissue in the body causing inflammation and tissue damage. SLE mainly damage nervous system, heart, joints, skin, lungs, blood vessels, kidneys, and liver. Disease progress is however unpredictable with periods of illness alternating with remissions. The prevalence of disease is 9 times higher in females than males. Studies show that SLE may have genetic link and there is no cure for it.








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