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Name Thyroglobulin ELISA kit
Price $228.00
Category NameAutoimmune Disease kits
Test96
MethodELISA: Enzyme Linked Immunosorbent Assay
PrincipleIndirect : Antigen Coated Plate
Detection RangeSemi-Quantitative elisa assay- Positive, Negative and Cut-off
Sample10ul serum
Specificity96.7%
Sensitivity100%
Total Time~75 min
Shelf Life12 months from the manufacturing date

Item #:                    1421-1   Quantity:               

 
   




 Description


Thyroglobulin ELISA Test kit





Thyroglobulin ELISA Test kit Intended Use
Thyroglobulin Enzyme-Linked Immunosorbent Assay (ELISA) is intended for the detection and semi-quantitation of antibodies to thyroglobulin in human sera. The results of this assay are to be used as an aid to the diagnosis of thyroid autoimmune disease.





Thyroglobulin ELISA Kit Components:
• Microplate: 96 wells coated with inactivated antigen
• HRP Conjugated goat anti-human IgG, IgA, IgM
• Serum Diluent Type III
• High Positive Control
• Low Positive Control
• Negative Control
• Calibrator
• Wash Buffer Type II (10X)
• TMB Chromogen/Substrate Solution
• Stop Solution (1N H2SO4)





Method Principle
This Diagnostic Automation/ Cortez Diagnostics Inc. Human Thyroglobulin test is an ELISA to detect IgG, IgA, and IgM antibodies to Thyroglobulin antigens. Purified Thyroglobulin antigens are attached to wells of a microplate. When antigens bound to the solid phase are brought into contact with a patient\\\\\\\'s serum, antigen specific antibody, if present, will bind to the antigen on the solid phase forming antigen-antibody complexes. For further details please refer to complete instructions manual supplied with the product.





Background Information
Thyroglobulin is a water soluble glycoprotein that is involved in the storage and synthesis of thyroid hormones. The thyroid microsomal antigen has been shown to be the enzyme thyroid peroxidase (TPO). Autoimmune thyroid disease is characterized by the presence of circulating autoantibodies directed to thyroid antigens. Hashimoto and Graves diseases are the best known of these diseases. The majority can be diagnosed by clinical presentation and their antibody profiles to thyroglobulin and TPO. Therefore, immunoassays for thyroid antibodies are useful for diagnostic evaluations of thyroid autoimmune disease. Antibodies to thyroglobulin and or TPO are present in most patients with goitrous thyroiditis (Hashimoto disease), atrophic thyroiditis (myxedema) and about 70-90% of Graves disease. Antibodies are also found in about half of the patients with primary hypothyroidism and thyrotoxicosis, and 10-20% of patients with simple goiters and thyroid tumors. There is also a relationship between thyroid antibodies and diabetes mellitus. Thyroid autoantibodies are present in about 6-7% of normal individuals and their incidence increases with age. ELISAs have been developed and validated for detecting autoantibodies to thyroid antigens providing great sensitivity, objective reading, and ease of use.





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