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Name Total Human IgE ELISA kit
Price $195.00
Category NameAllergy ELISA kits
MethodELISA: Enzyme Linked Immunosorbent Assay
PrincipleSandwich Complex
Detection Range0- 800IU/ml
Sample20ul serum
Sensitivity5 IU/mL
Total Time80min
Shelf Life12 Months from the manufacturing date

Item #:                    1801-18   Quantity:               



Human Total IgE ELISA kit

Total IgE ELISA kit Intended Use
Human Total IgE ELISA kit provides a rapid, sensitive, and reliable assay for the quantitative determination of total Immunoglobulin E (IgE) in human serum.

Total IgE ELISA kit Components:
• Anti- IgE Coated Microwell (12x8 strips)
• IgE Zero Buffer
• Enzyme Conjugate Reagent
• Reference Standard Set
• TMB Reagent
• Stop Solution (1N HCl)

Limitation of Procedure
No single assay should be used as the only basis for arriving at a diagnostic

Test Method Principle
This Diagnostic Automation/ Cortez Diagnostics Inc. Human Allergen Total IgE ELISA Test is based on the principle of solid phase sandwich ELISA assay using allergen coated microwells. For additional information and details please refer to the instructions for use.

Background Information
Total immunoglobulin E (IgE) levels in blood increases in patients with atopic asthma, allergies, dermatitis, and hay fever. This elevation in IgE level may be indicative of an increased probability of an IgE-mediated hypersensitivity, responsible for allergic reactions. Moreover, parasitic infestations such as hookworm, and certain clinical disorders including aspergillosis, have also been demonstrated as contributors for elevation of IgE. The serum IgE concentration in a patient is dependent on both the extent of the allergic reaction and the number of different allergens to which the patient is sensitized. Nonallergic individuals have IgE concentrations that vary widely and increase steadily during childhood, reaching their highest levels at age 15 to 20, and then remaining constant until about age 60 when its level slowly declines. Decreased levels of IgE have been reported in cases of hypogammaglobulinemia, autoimmune diseases, malaria, ulcerative colitis, hepatitis, and cancer.
For the diagnosis of allergic conditions a variety of tests exist, including skin and blood testing. Diagnostic Automation/Cortez Diagnostics blood test ELISAs are accurate measurements which can be used for quantitative detection of IgE, IgM, IgA, or IgG specific mediated responses to known allergens. These ELISA based testing allows for the accurate detection of hypersensitive-driven responses to known allergens of interest. We offer multiple stock allergens and we have the capacity to generate custom made microwell plates upon request.

Diagnostic Automation/ Cortez Diagnostics Inc. also provides other Human Allergen Specific ELISA kits: Human Allergen Specific IgA ELISA Test kit, Human Allergen Specific IgM ELISA Test kit, and Human Allergen Specific IgG ELISA. Additionally, Total Human ELISA Kits are available: Total Human IgA ELISA Test kit, Total Human IgM ELISA Test kit, Total Human IgG ELISA Test kit, and Total Human IgE ELISA Test kit. For more information about ELISA Kits, Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representatives at 818-591-3030.