Description

Human growth hormone (HGH) ELISA Test kit




HGH ELISA Test kit Intended Use
The HGH ELISA is intended for the quantitative determination of human growth hormone (HGH) concentration in human serum. The test is useful in the diagnosis and treatment of disorders involving the anterior pituitary gland.




HGH ELISA Kit Components:
• Microplate: 96 wells coated with sheep anti-hGH
• Reference standard set
• Enzyme conjugate reagent
• TMB Substrate
• Stop Solution




Method Principle
This Diagnostic Automation/ Cortez Diagnostics Inc. HGH ELISA is based on the principle of a solid phase enzyme-linked immunosorbent assay (ELISA). The assay system utilizes a sheep anti-HGH antibody for solid phase immobilization and a mouse monoclonal anti-HGH antibody in the antibody enzyme-HRP conjugate solution. For further details please refer to complete instructions manual supplied with the product.




Background Information
HGH or somatotropin is a polypeptide secreted by the anterior pituitary. HGH measurement is primarily of interest in the diagnosis and treatment of various forms of abnormal growth hormone secretion. Disorders caused by hypo-secretion include dwarfism and unattained growth potential, and hyper-secretion is associated with gigantism and acromegaly. Caution must be exercised in the clinical interpretation of growth hormone levels. These vary throughout the day, making it difficult to define a normal range or to judge an individual status based on a single determination. Many factors are known to influences the rate of growth hormone secretion, including periods of sleep and wakefulness, exercise, stress, hypoglycemia, estrogens, corticosteroids and L-dopa. Various challenge tests have therefore been devised to differentiate them. For example, with the onset of deep sleep or after 15 to 20 minutes of vigorous exercise, growth hormone levels normally rise.



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