Description

PR-3 ELISA Test kit





PR-3 ELISA Test kit Intended Use
Proteinase-3 (PR-3) Enzyme-Linked Immunosorbent Assay (ELISA) is intended for the detection and semi-quantitative determination of antibodies to PR-3 in human sera. The results of the assay may be used as an aid in the diagnosis of Wegener’s granulomatosis.






PR3 ELISA Kit Components:
• Microplate: 96 wells coated with Proteinase-3 antigen
• HRP Conjugated Goat antihuman IgG, IgA, and IgM
• Serum Diluent Type III
• High Positive Control
• Low Positive Control
• Negative Control
• Calibrator
• Wash Buffer Type II (20X)
• Chromogen/Substrate Solution Type II (TMB)
• Stop Solution (1N H2SO4)





Method Principle
This Diagnostic Automation/ Cortez Diagnostics Inc. Human PR-3 test is an Enzyme-Linked Immunosorbent Assay to detect IgG, IgA, and IgM antibodies to PR-3 antigens. Purified PR-3 antigens are attached to microplate wells. Diluted test sera are added to each well. If the antibodies are present that recognize the antigen, antigen-antibody complexes are formed. For further details please refer to complete instructions manual supplied with the product.





Background Information
Anti-neutrophil cytoplasmic antibody (ANCA) are a group of autoantibodies. Two major fluoroscopic patterns can be recognized: a diffuse cytoplasmic staining (C-ANCA), and a perinuclear/nuclear staining (P-ANCA). In patients with vasculitis, more of 90% of C-ANCA are directed against proteinase 3 (PR3-ANCA) whereas approximately 80-90% of P-ANCA recognize myelperoxidase (MPO-ANCA). Testing for both P-ANCA and C-ANCA is highly recommended in the laboratory workup of patients who present with clinical features suggestive of SV. The clinical syndromes most frequently associated with ANCA are as follows: Wegener’s granulomatosis, Polyarteritis, “Overlap” Vasculitis, Idiopathic Crescentic Glomerulonephritis (ICGN), and Kawasaki Disease.




For more information about ELISA Kits, Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representative at 818-591-3030.