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Name Rheumatoid Factor (RF) ELISA kit
Price $410.00
Category NameAutoimmune Disease kits
MethodELISA: Enzyme Linked Immunosorbent Assay
PrincipleELISA -Indirect; Antigen Coated Plate
Detection RangeQualitative: Positive, negative, cut off
Sample10ul serum
Total Time~65 min
Shelf Life12 Months from the manufacturing date

Item #:                    2550-1   Quantity:               



RF ELISA Test kit

RF ELISA Test kit Intended Use
Rheumatoid Factor (RF) Enzyme-Linked Immunosorbent Assay (ELISA) is intended for the qualitative detection of IgM antibodies in human serum to RF antigen and as an aid in the diagnosis of rheumatoid arthritis.

RF ELISA Kit Components:
• Microplate: 96 wells coated with Purified Rheumatoid Factor IgG antigen
• HRP Conjugated goat anti-human IgG
• Serum Diluent Type II
• Positive Control
• Negative Control
• Calibrator
• Wash Buffer Type I (20X)
• Chromogen/Substrate Solution Type I
• Stop Solution (1N H2SO4)

Method Principle
This Diagnostic Automation/ Cortez Diagnostics Inc. Human Rheumatoid Factor test kit uses the ELISA technique for the detection of IgM antibodies to IgG antigen. The purified antigen is bound to microplate wells. Patient serum samples to be assayed for antibody are first diluted and added to each well. If antibody is present in the patient serum, antigen antibody complexes are formed. For further details please refer to complete instructions manual supplied with the product.

Background Information
Rheumatoid Factors (RFs) are immunoglobulins of any isotype with antibody activity directed against antigenic sites on the Fc portion of IgG molecules. Because of its pentavalent structure and ability to cross-link immunoglobulin G antigen, IgM-RF is the main isotype identified by clinically available diagnostic assays for RF detection. Rheumatoid factors may exist as the mu, gamma, alpha, and epsilon isotypes. Rheumatoid factors are found in 1 to 4% of the general population, present in 75% of adult patients with the highest incidence of rheumatoid factors occurring in persons over 65 years of age, and nearly all patients with Felty and Sjogren syndrome.

For more information about ELISA Kits, Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representative at 818-591-3030.