Aflatoxin M1 ELISA Test kit




Aflatoxin M1 ELISA Test kit Intended Use
Aflatoxin M1 Food ELISA Kit that is an Enzyme Immunoassay for the Quantitative determination of Aflatoxin M1 in Food. This Aflatoxin M1 Food ELISA Kit is a quick, economical, and sensitive Food Toxin ELISA Kit for detection of Aflatoxin M1 in food.




Aflatoxin M1 ELISA Kit Components:
• Microplate: 96 wells coated with aflatoxin conjugate
• Aflatoxin M1 Standards
• Anti-Aflatoxin M1 Antibody
• HPR-Enzyme Conjugate anti-rabbit-IgG
• Substrate Solution
• Stop Solution
• Sample/Standard Diluent
• Washing Solution




Method Principle
This Diagnostic Automation/ Cortez Diagnostics Inc. Aflatoxin M1 Food ELISA Kit is a quantitative test based on the principle of the enzyme linked immunosorbent assay. An aflatoxin conjugate is bound on the surface of a microplate wells. Immobilized and free aflatoxin M1 compete for the antibody binding sites. For further details please refer to complete instructions manual supplied with the product.




Background Information
Out of about 20 known aflatoxins, the moulds Aspergillus flavus and A. parasiticus produce exclusively aflatoxin B1, B2, G1 and G2, and all the other aflatoxins are derivates of these four. Aflatoxins belong to the class of mycotoxins which are the strongest mycotoxins, acting primarily as a hepatotoxic and carcinogen. The four main aflatoxins show a different toxicity. B1 is without doubt the most toxic aflatoxin, followed by G1, B2 and G2. Aflatoxins cause both chronic and acute intoxications. Contaminations with aflatoxins occur mostly with nuts and grain. Aflatoxin M1 was the first metabolite of Aflatoxin B1, which could unequivocally be detected in the milk of cows in 1963. Thus this first derivative was called Aflatoxin M1 (Milk). As further investigations showed, also other mammalians excrete Aflatoxin M1 in milk, feces and urine. Contaminations of milk and milk products can be hazardous for human beings, because M1 is similar to Aflatoxin B1 regarding its hepatotoxicity, but M1 is only less carcinogenic. This Aflatoxin M1 ELISA kit is a quick, economical, and sensitive method to detect Aflatoxin M1 in Milk and Milk Products.



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