Chloramphenicol ELISA Test kit





Chloramphenicol ELISA Test kit Intended Use
This Chloramphenicol Food ELISA Kit that is an Enzyme Immunoassay for the Quantitative determination of Chloramphenicol in Food. This is a quick, economical, and sensitive Food Toxin ELISA Kit which is designed to detect Chloramphenicol in food.




Chloramphenicol ELISA Kit Components:
• Microplate: 96 wells coated with antibody binding protein
• Chloramphenicol Standards
• Conjugate Chloramphenicol-peroxidase
• Anti-Chloramphenicol Antibody
• Sample diluent
• Substrate Solution, TMB
• Stop Solution
• Washing Solution





Method Principle
This Diagnostic Automation/ Cortez Diagnostics Inc. Chloramphenicol Food ELISA Kit is a quantitative test based on the principle of the enzyme-linked immunosorbent assay. An antibody binding protein is coated on the surface of a microtiter plate. Chloramphenicol containing samples or standards and an antibody directed against chloramphenicol are given into the wells of the microtiter plate. The chloramphenicol contained in samples or standards will bind to the antibody which reacts with the binding protein. For further details please refer to complete instructions manual supplied with the product.





Background Information
Due to its outstanding antibacterial properties, chloramphenicol is an often used antibiotic in the production of milk, meat and eggs. In humans it leads to haematotoxic side effects such as chloramphenicol induced aplastic anemia. This has caused low limits in Germany, e.g. 1 I¼g/kg for milk, meat and eggs. Until now, the chloramphenicol concentration was determined by radioimmunoassay or by gas chromatography. However, compared with conventional methods, ELISA shows some essential advantages. Forty samples on this Food ELISA test kit can be assayed in duplicate within 60 minutes.



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