Lupine ELISA Test kit




Lupine ELISA Test kit Intended Use
Lupine ELISA Kit that is an Enzyme Immunoassay for the quantitative determination of Lupine/Lupin in Food. This Lupine ELISA Kit is a rapid and sensitive Food Allergen ELISA Kit which is designed to provide a sensitive and convenient method for screening food samples for lupine/lupin residues. This Lupine ELISA Kit represents a highly sensitive Food Allergen ELISA Kit detection system for lupine and is particularly capable of the quantification of residues in sausage, bakery products, potato products, ketchup, and juices.




Lupine ELISA Kit Components:
• Microplate: 96 wells coated with anti-lupine antibodies
• Extraction and sample dilution buffer
• Lupine Standards
• Conjugate anti-lupin-peroxidase
• Substrate Solution, TMB
• Stop Solution
• Washing Solution




Method Principle
This Diagnostic Automation/ Cortez Diagnostics Inc. Food Lupine Food ELISA Kit is a quantitative test based on the principle of the enzyme linked immunosorbent assay. An antibody directed against lupine proteins is bound on the surface of a microplate wells. For further details please refer to complete instructions manual supplied with the product.




Background Information
Lupine/Lupin which belongs to the legume family has a very high protein content of about 35-45%. Some of these proteins are known for being allergenic, due to cross-reaction to allergens of soy and peanut. Because of its convenient nutritional properties the use of lupine flour as a food ingredient is highly increasing. Lupine is often used in dietary products, especially for milk allergic people, vegetarians, and patients with celiac disease. For lupine allergic persons, hidden lupine allergens in food are a critical problem, since very low amounts of lupine can cause allergic reactions, leading to anaphylactic shock in severe cases. Thus, sensitive detection systems, such as this Lupine ELISA Kit for detecting lupine residues in foods are important.




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