Mustard ELISA Test kit




Mustard ELISA Test kit Intended Use
Mustard ELISA Kit that is an Enzyme Immunoassay for the quantitative determination of Mustard in food. This Mustard ELISA Kit represents a highly sensitive Food Allergen ELISA Kit detection system for yellow mustard and is particularly capable of the quantification of resides in sausage, dressings, soups, cheese, and mixed herbs.




Mustard ELISA Kit Components:
• Microplate: 96 wells coated with anti-mustard antibodies
• Extraction and sample dilution buffer
• Mustard Standards
• Conjugate anti-mustard-peroxidase
• Substrate Solution, TMB
• Stop Solution
• Washing Solution




Method Principle
This Diagnostic Automation/ Cortez Diagnostics Inc. Food Mustard Food ELISA Kit is a quantitative test based on the principle of the enzyme linked immunosorbent assay. An antibody directed against mustard proteins is bound on the surface of a microplate wells. For further details please refer to complete instructions manual supplied with the product.





Background Information
Mustard belongs to the Brassica plants and with very high protein content ranging about 30-35%. Some of these proteins are known for being allergenic. These proteins are predominantly heat resistant making them stable to different production processes. In addition to brown and black mustard, yellow mustard is used as an ingredient in many foods and food preparations. For mustard allergic persons, hidden mustard allergens in food are a critical problem. Already very low amounts of mustard can cause allergic reactions, which may lead to anaphylactic shock in severe cases. Cross-contamination, mostly in consequence of the production process, is often noticed. The sausage production process is a representative example. This explains why in many cases the existence of mustard residues in food cannot be excluded. For this reason sensitive detection systems, like this Mustard ELISA Kit, for mustard residues in foodstuffs are required. This Mustard ELISA Kit is a rapid and sensitive Food Allergen ELISA Kit which is designed to provide a sensitive and convenient method for screening food samples for mustard residues. Due to high cross reactivity, this Mustard ELISA test is also suitable for the detection of brown mustard and black mustard.





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