Walnut ELISA Test kit




Walnut ELISA Test kit Intended Use
The walnut ELISA Kit is a quantitative test based on the principle of the enzyme linked immunosorbent assay. This Walnut ELISA represents a highly sensitive detection system and is particularly capable of the quantification of walnut residues in cookies, cereals, ice cream and chocolate.





Food walnut ELISA Kit Components:
• Microplate: 96 wells coated with anti-walnut antibodies
• Walnut Standards
• Conjugate anti-walnut-peroxidase
• Substrate Solution
• Extraction and sample dilution buffer
• Stop Solution
• Washing Solution





Method Principle
This Diagnostic Automation/ Cortez Diagnostics Inc. Food walnut ELISA Kit is a quantitative test based on the principle of the enzyme linked immunosorbent assay. Walnut quantitative test is based on the principle of the enzyme linked immunosorbent assay. An antibody directed against walnut proteins is bound on the surface of a microplate. Walnut containing samples or standards are given into the wells of the microplate. For further details please refer to complete instructions manual supplied with the product.




Background Information
Walnut is high in protein with15% the fraction of its content. Some of these proteins are known for being allergenic, such as rJug r 1 and rJug r 4. Many of them are heat resistant making them stable to different production processes. For this reason walnut represents an important food allergen. Hidden walnut allergens in food are a critical problem in people with hidden walnut allergy. Already very low amounts of walnut can cause allergic reactions, which may lead to anaphylactic shock in severe cases. Because of this, walnut allergic persons must strictly avoid the consumption of walnuts or walnut containing food. Cross-contamination, mostly in consequence of the production process, is often noticed. The chocolate production process is a representative example. This explains why in many cases the existence of walnut residues in food cannot be excluded. For this reason sensitive detection systems for walnut residues in foodstuffs are required. This Walnut ELISA offers a highly sensitive detection system to detect walnut residues in food.




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