Zearalenone ELISA Test kit





Zearalenone ELISA Test kit Intended Use
The zearalenone ELISA Kit is a quantitative test based on the principle of the enzyme linked immunosorbent assay. This Zearalenone ELISA is a quick, economical, and sensitive method to detect zearalenone in food.





Food zearalenone ELISA Kit Components:
• Microplate: 96 wells coated with anti-walnut antibodies
• Walnut Standards
• Conjugate anti-walnut-peroxidase
• Substrate Solution
• Extraction and sample dilution buffer
• Stop Solution
• Washing Solution





Method Principle
This Diagnostic Automation/ Cortez Diagnostics Inc. Food zearalenone ELISA Kit is a quantitative test is based on the principle of the enzyme linked immunosorbent assay. A zearalenone conjugate is bound on the surface of a microtiter plate. Zearalenone containing samples or standards and an antibody directed against zearalenone are given into the wells of the microtiter plate. Immobilized and free zearalenone compete for the antibody binding sites. For further details please refer to complete instructions manual supplied with the product.




Background Information
Zearalenone as a mycotoxins is a degradation product of the secondary metabolism. It is produced by different molds of the Fusarium gender. These molds infect grain and other types of foods such as peanuts and beans already during their growth. When a considerable amount of zearalenone contaminated feed is taken up by cows, it can also be detected in their milk, and it even can be found in beer. Zearalenone shows a strong estrogen-like activity. Thus zearalenone can cause an enlargement of the uterus, diminution of the ovarian glands and even infertility. Zearalenone is one of the main contaminants of farm products, which can be taken up by humans and animals. In order to protect humans of illnesses caused by zearalenone, a qualitative and quantitative control of threatened food is essential, together with appropriate hygienic measures, which help to prevent the development of zearalenone.



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