Luteinizing Hormone (LH) ELISA Test kit




LH ELISA Test kit Intended Use
The LH ELISA Kit is for the quantitative determination of luteinizing hormone (LH) in human serum. This assay is useful in the diagnosis and treatment of gonadal dysfunction.




LH ELISA Kit Components:
• Microplate: 96 wells coated with monoclonal anti-α-LH antibody
• Enzyme Conjugate Reagent
• Reference Standard Set
• TMB Reagent
• Stop Solution




Method Principle
This Diagnostic Automation/ Cortez Diagnostics Inc. LH ELISA is based on the principle of a solid phase enzyme-linked immunosorbent assay. The assay system utilizes a mouse monoclonal anti-α-LH antibody for microplate wells immobilization, and a mouse monoclonal anti-β-LH antibody in the antibody-enzyme (horseradish peroxidase) conjugate solution. For further details please refer to complete instructions manual supplied with the product.




Background Information
LH is produced in both men and women from the anterior pituitary gland in response to luteinizing hormone-releasing hormone (LH-RH or Gn-RH), which is released by the hypothalamus. A lack of secretion by the anterior pituitary may cause lower LH levels. As may be expected, low levels may result in infertility in both males and females. Low levels of LH may also be due to the decreased secretion of GnRH by the hypothalamus. Low LH values may therefore indicate some dysfunction of the pituitary or hypothalamus, but the actual source of the problem must be confirmed by other tests. LH is also called interstitial cell-stimulating hormone (ICSH) in men. In the differential diagnosis of hypothalamic, pituitary, or gonadal dysfunction, assays of LH concentration are routinely performed in conjugation with follicle-stimulating hormone (FSH) assays since their roles are closely interrelated. Furthermore, the hormone levels are used to determine menopause, pinpoint ovulation, and monitor endocrine therapy.



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