T4 ELISA Test kit



T4 ELISA kit Intended Use:
Thyroxine (T4) in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of total T4 in Human serum. This test is useful for diagnosis and treatment of thyroid disorders such as hypothyroidism and hyperthyroidism.




T4 ELISA kit Components:
• Microplate: Antibody-Coated Wells
• Enzyme Conjugate Concentrate
• Enzyme Conjugate Diluent
• Reference Standard set
• TMB Reagent
• Stop Solution (1N HCl)




Test Method Principle:
This Diagnostic Automation/ Cortez Diagnostics Inc. the T4 ELISA test kit, antibody to T4 is coated on a microplate wells. This test is based on the principle of competitive immunoassay. T4 in the patient sample competes with the added enzyme-labeled T4 for the limited binding sites on the T4 antibody. Separation of the unbound T4 from antibody-bound T4 and the subsequent measurement of the labeled fraction of the bound phase complete the test. For additional information and details please refer to the instructions for use.




Background Information:
Thyroid hormones are extremely important and have diverse actions. Under- or over-production of these hormones has potent effects. Triiodothyronine (T3) & T4 are the two main hormones produced by the thyroid gland and Iodine is necessary for their production. A deficiency of iodine leads to decreased production of T3 & T4 enlarges the thyroid tissue and will cause the disease known as Goitre. The production of T3 and T4 by thyroid is regulated by the pituitary hormone (thyroid stimulating hormone, TSH) which in turn is also regulated by the hypothalamus TSH Releasing Hormone (TRH).
Separate blood tests can be performed to measure the free T4 hormone in the blood. Both total and free T4 test may be tested when a patient has an abnormal TSH and/or T3 test result. The Thyroid Panel combines T3, T4, and TSH which are some of the most common markers today. A total thyroid test workup including T3, T4 and TSH may be ordered in thyroid related disease.



Diagnostic Automation/ Cortez Diagnostics Inc. also provide other thyroid kits: T3 ELISA kit, T4 ELISA kit, TSH ELISA kit, TSH Ultra-sensitive ELISA kit, Free T3 ELISA kit, Free T4 ELISA kit and T Uptake ELISA kit. For more information about ELISA Kits, Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representatives at 818-591-3030.